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特性

應(yīng)用

Strep-（WSHPQFEK）標(biāo)簽蛋白的高特異性親和純化

特異性

與Strep-tagged (WSHPQFEK) 蛋白親和

結(jié)合載量

>7?mg/ml

配體

StrepTactin^®

粒徑

30 μm

基材

6% 交聯(lián)瓊脂糖

充柱量

原包裝含5%保存緩沖液

化學(xué)穩(wěn)定性

2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol

短期保存

中性緩沖液（詳見操作指南Protocol）

長期保存

4°C，20%乙醇

PURIFIED PROTEIN

YEAR

AUTHOR

NPTX1

2020

Sticco M.J., Palomino P.A.P., Lukacsovich D., Thompson B.L., F?ldy C., Ressl S., Martinelli D.C.

Sarm1

2021

Li Y., Pazyra-Murphy M.F., Avizonis D., de Sá Tavares Russo M., Tang S., Chen C-Y., Hsueh Y-P., Bergholz S.J., Jiang T., Zhao J. J:, Zhu J., Woo Ko K., Milbrandt J., DiAntonio A., Segal R.A.

NMNAT

2022

Dingwall C.B ., Strickland A., Yum S.W., Yim A.K., Zhu J., Wang P.L., Yamada Y., Schmidt R.E, Sasaki Y., Bloom A.J., DiAntonio A., Milbrandt J.

TIR domains and TIR domain-encoding proteins

2022

Weagley J.S., Zaydman M., Venkatesh S., Sasaki Y., Damaraju N., Yenkin A., Buchser W., Rodionov D.M. Osterman A., Ahmed T., Barratt M.J., DiAntonio A., Milbrandt J., Gordon J.I.